PROTEIN CHARACTERISATION

Crystal screening
Molecular weights
Melting points

Zetasizer nano the ultimate in simplicity

The Malvern Zetasizer-nano is a multi purpose analyser combining simplicity and precision. This desktop system combines the capability of static and dynamic light scattering (SLS and DLS) plus Zeta potential. Novel optics provides exceptional sensitivity to size molecules smaller than 1000 Dalton's. Sizing of nano-particles from 0.6 to 6000nm is now extremely simple and rapid.

Rapid Crystal screening

The Zetasizer-nano can dramatically reduce the screening time needed to optimise protein crystal growth. SLS can be used to determine the second viral coefficient (A2), a property that describes the strength of protein-solvent interaction and correlates with solubility. The determination of A2 for various protein solutions can help predict which is the most suited to facilitate crystal growth. DLS is complimentary since it is ideal for distinguishing which solutions are unlikely to yield crystals due to impurities or non-specific aggregates.

Easy molecular weight

Since the majority of bio-molecules and polymers are less than 100nm, molecular weight can be determined by DLS. If absolute molecular weight is required measurements are made at a number of concentrations. However for fast and easy screening between monomers and dimers only a single measurement is needed. The technique is very sensitive to small changes in protein size, enabling detection of small conformational changes.

Disposable Zeta cell

Dual parameter melting points

Trend measurements of size or zeta potential as a function of temperature allow for the determination of protein melting points and can give information on heat stability and conformational change. The temperature range is 2 to 90 degrees C.

Zeta potential

Zeta potential is measured by the patented M3-PALS technology, giving the highest ever sensitivity and resolution. Cross contamination is eliminated by means of a unique disposable capillary cell.

Chromatography detector

A new chromatography flow-mode for the Zetasizer-nano enables it to be also used as a highly sensitive size exclusion chromatography detector. This enhanced capability provides a major new tool for protein and other biomolecular research.

This new capability is available to existing Zetasizer-nano customers.
Simply purchase a flow cell and download a free software upgrade.

chromatography flow-mode combines dynamic light scattering with size exclusion chromatography in order to perform Absolute Size Exclusion Chromatography. This technique allows protein size to be measured directly without the need for column calibration. Molecular weight standards are no longer required to identify oligomeric species or to determine protein purity.

The flow cell in the Zetasizer is connected after the last detector in a size exclusion chromatography system. The software makes the entire measurement process simple and straightforward. For the majority of protein separations no extra parameters are required. Size is plotted in real time as material is eluted from the column. The molecular weight of each peak is calculated automatically at the end of the measurement.

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