SURFACE PLASMON RESONANCE SPR

Low cost personal analyser

Now quantitative Surface Plasmon Resonance (SPR) analysis is available to labs on a limited budget. The SensiQ provides quality data at an affordable price. It retains many of the advantages of expensive instruments such as low noise, accurate reference curve subtraction and well-known surface chemistry.

SensíQ is a dual-channel, semi-automated SPR system that provides high quality kinetic, affinity and concentration data. The design combines flow injection analysis, microfluidics and proven surface chemistry. Intuitive data analysis software simplifies the analysis of binding interactions so that non-expert users can extract kinetic and affinity constants in minutes.

Sensors

SensíQ utilises disposable spreeta sensors with Kretschmann SPR geometry. They are provided with a planar carboxylate surface and a variety of immobilisation protocols. The sensors are proven to be highly sensitive and quick and easy to install. The dual 50 nanolitre flow cells have been optimised for mass transport of analyte to the interaction surface with minimum dispersion and dead volume.

Software

The control software acquires data from both sensing channels as raw response curves. The reference channel data is subtracted in real time to provide clean quality response curves. Binding responses are determined by selecting report points in the response curves. Tables of report points can be created then recalled for each assay and the entire analysis is tracked with an event log.

The acquired data is processed within minutes using Qdat analysis software, which is based on the proven Clamp and Scrubber architecture. The program guides the user through the simple steps required to obtain kinetic and affinity constants and concentration.

Applications

Measurements inherent in SPR are kinetics of association and dissociation, affinity constants, concentration and ligand fishing. Affinity pairs include:

• Protein-Protein
  
• Protein-DNA
  
• Protein-Small Molecule
  
• Antibody-Antigen
  
• Membrane receptor-Ligand
  
• Peptide-Receptor
  

Site Map

Particle Sizing Rheology & Mixing Micro Emulsions Surface Interactions Pharmaceutical Biotech - Protein Spectroscopy Rapid Kinetics