This unique instrument is specifically designed for the detection of rapid kinetics. It can be easily integrated with the Bio-Logic stopped flow-quenched flow equipment, under the control of the same computer and software. This combination provides a very powerful kinetics analysis system, with high sensitivity and very fast data acquisition.

The Spectrometer can be configured to measure reactions by UV absorbance, fluorescence, or light scattering. There is also a dual detection mode for recording UV absorbance plus fluorescence, or two different fluorescence wavelengths. Fibre optics link the spectrometer excitation and emission monochromators to the external stop flow observation cuvette.

Fast kinetics are observed at fixed wavelength, with the data acquisition period selectable from 50 milliseconds to 1000 seconds. The system can also be operated as a normal fluorescence spectrometer with exitation and emission wavelength scans.

Fibre Optics Link Stop Flow to Multi Mode Spectrometer

Fluorescence detected refolding of lysozyme at only 3 µg/ml. This shows that kinetics with as few as 100ng/ml may be recorded.